System.InvalidoPerationException: no Owin.Environment Item не было обнаружено в контексте.
Most likely it cannot find the OWIN Startup class. The default convention for the Startup class is [AssemblyName].Startup. If you're no longer following that convention you'll need to specify the full name of your Startup class in the Web.Config.
The next release of Microsoft.Owin.Host.SystemWeb package now throws detailed exception messages when the Startup class cannot be found.
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